Magnetic Lateral Flow Strip for the Detection of Cocaine in Urine by Naked Eyes and Smart Phone Camera
نویسندگان
چکیده
Magnetic lateral flow strip (MLFS) based on magnetic bead (MB) and smart phone camera has been developed for quantitative detection of cocaine (CC) in urine samples. CC and CC-bovine serum albumin (CC-BSA) could competitively react with MB-antibody (MB-Ab) of CC on the surface of test line of MLFS. The color of MB-Ab conjugate on the test line relates to the concentration of target in the competition immunoassay format, which can be used as a visual signal. Furthermore, the color density of the MB-Ab conjugate can be transferred into digital signal (gray value) by a smart phone, which can be used as a quantitative signal. The linear detection range for CC is 5-500 ng/mL and the relative standard deviations are under 10%. The visual limit of detection was 5 ng/mL and the whole analysis time was within 10 min. The MLFS has been successfully employed for the detection of CC in urine samples without sample pre-treatment and the result is also agreed to that of enzyme-linked immunosorbent assay (ELISA). With the popularization of smart phone cameras, the MLFS has large potential in the detection of drug residues in virtue of its stability, speediness, and low-cost.
منابع مشابه
Development of a Lateral Flow Immunoassay Using Recombinant Dense Granular Antigen (GRA) 7 to Detect Anti-Toxoplasma gondii IgG Antibodies
Toxoplasma gondii is an intracellular protozoan parasite that causes toxoplasmosis and is of medical importance in pregnant women and immunosuppressed patients. In recent years, many methods have been developed for the detection of infection caused by this parasite; however, most of the developed methods are not adequately sensitive. The dense granular antigen (GRA) ...
متن کاملSilent Cocaine Poisoning
Background: Cocaine poisoning is known for causing severe clinical effects such as tachycardia, hypertension, agitation and confusion. Absence of clinical manifestations in cocaine poisoning is unusual. Case report: A 26-year old man, known to be a cocaine addict, declared that he was forced by cocaine dealers to swallow many tablets of cocaine, six hours prior to admission to emergency departm...
متن کاملSmart-phone attachable, flow-assisted magnetic focusing device
Detection and sorting of particles and cells in a continuous flow stream is of great importance to downstream biological studies and high-throughput screening. Many particle separation techniques require peripheral devices, complex channel designs or setups that can limit the process and its applications. Here, we present a smart-phone attachable continuous flow magnetic focusing device as an i...
متن کاملCellular Phone-Based Image Acquisition and Quantitative Ratiometric Method for Detecting Cocaine and Benzoylecgonine for Biological and Forensic Applications
Here we describe the first report of using low-cost cellular or web-based digital cameras to image and quantify standardized rapid immunoassay strips as a new point-of-care diagnostic and forensics tool with health applications. Quantitative ratiometric pixel density analysis (QRPDA) is an automated method requiring end-users to utilize inexpensive (∼ $1 USD/each) immunotest strips, a commonly ...
متن کاملInteraction Control Based on Vision for AR Interface of Smart Phone
This paper proposes methods for detecting and tracking moving body parts such as the eyes, lips, and hands to enable natural interaction with smart phone platforms. For demonstrating these methods we have implemented a markerless-based augmented reality on an Android-based smart phone. For interaction the user does not use any extra appliances except for the natural movement of the eyes, lips a...
متن کامل